Dr Melania Lo Iacono
Term-contract as Researcher with experience in molecular
diagnosis of hematological rare diseases Hematology and rare blood diseases division of Ospedali Riuniti Villa Sofia- Cervello Hospital, Palermo, Italy.Faculty Member
Excellent organizational and interpersonal skills acquired during different workshops organized at the Department of Experimental Biomedicine and Clinical Neurosciences, Section of Human Anatomy , University of Palermo .
Excellent interpersonal skills with students acquired during laboratories organized for students of the faculty of Medicine, of University of Palermo, at the Department of Experimental Biomedicine and Clinical Neurosciences , Section of Human Anatomy.
Skills in the use electrophoretic techniques: (proteins and DNA extraction kit), thermal cycler, incubator, spectrophotometer, tools for cell culture (laminar flow hood, centrifuges, chamber Burker). Techniques acquired: immunohistochemistry , immucitochemistry, histological staining, protein extraction, Western blotting, DNA and RNA extraction, PCR, RealTime-PCR and RT–PCR,, southern blotting, RFLP analysis with restriction enzymes, two-dimentional electrophoresis, mixed lymphocyte reaction, flow cytometry analysis, transduction, lentiviral preparation, real-time PCR.
Cell culture: isolation and characterization of umbilical cord-derived mesenchymal stem cells. Several differentiation protocol toward hepatic-like phenotype, adipocyte-like, osteocyte-like, chondrocyte-like phenotype. isolation of hematopoietic stem cells, isolation and separation of CD34+ cells by magnetic beads (Miltenyi), flow cytometry analysis by ISHAGE methods, colony forming unit assay, real time- PCR, preparation of lentiviral vector.